Alternative Names:

GFP ; yfp ; Green fluorescent protein ;

Application:

WB

Reactivity:

All

Dilution:

WB 1:3000~1:10000

Western blot analysis of GFP fusion protein expression in (1) 293T cell lysate; (2) 293T cell transfected GFP fusion protein with GFP Antibody(HRP conjugated).

Purification:

Affinity-chromatography

Specificity:

GFP Antibody(HRP conjugated) detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.

Immunogen:

A synthesized peptide

Description:

Green fluorescent protein (GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.