Alternative Names:
GFP ; yfp ; Green fluorescent protein ;
Application:
WB IHC ICC/IF
Reactivity:
All
Dilution:
WB 1:5000~1:10000 IHC 1:100~1:200 ICC/IF 1:100~1:200
Western blot analysis of GFP expression in 293 cell lysate transfected with GFP. |
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| Immunohistochemical analysis of paraffin-embedded GFP transgenic mouse liver, using GFP Antibody. |
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Purification:
Affinity-chromatography
Specificity:
GFP antibody detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.
Immunogen:
A synthesized peptide derived from green fluorescent protein
Description:
Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
