Alternative Names:

N/A

Application:

WB IHC ICC/IF

Reactivity:

H M R Mk Dg Ch Pg Rabbit

Dilution:

WB 1:3000~1:10000 IHC 1:100~1:200 ICC/IF 1:100~1:200

Western blot analysis of beta Actin expression in (1) Hela cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate with beta Actin mouse mAb
Immunohistochemical analysis of paraffin-embedded human heart, using beta Actin Mouse Monoclonal Antibody.
Immunofluorescent analysis of HepG2 cells, using beta Actin mAb.

Purification:

Affinity-chromatography

Specificity:

beta Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

Immunogen:

A synthesized peptide derived from human beta Actin

Description:

betaactin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -25°C long term. Avoid freeze / thaw cycle.