Alternative Names:

Apoptosis signal- regulating kinase 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen-activated protein kinase kinase kinase 5

Application:

WB  IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000    IHC 1:50-1:200

Western blot analysis of ASK1 phosphorylation expression in TNF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-ASK1 (Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Serine 966

Immunogen:

A synthesized peptide derived from human ASK1 around the phosphorylation site of Serine 966

Description:

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.