Alternative Names:

5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK1; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; A

Application:

WB  IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000    IHC 1:50-1:200

Western blot analysis of AMPK1 phosphorylation expression in heatshock treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-AMPK1 (Ser485) Antibody detects endogenous levels of AMPK1 only when phosphorylated at Serine 485

Immunogen:

A synthesized peptide derived from human AMPK1 around the phosphorylation site of Serine 485

Description:

AMPKA1 a protein kinase of the CAMKL family that plays a central role in regulating cellular and organismal energy balance in response to the balance between AMP/ATP, and intracellular Ca(2+) levels.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.