Alternative Names:

5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; AMPK; AMPK subunit alpha-2; AMPK-alpha-2 chain; AMPK2; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit

Application:

WB  IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000    IHC 1:50-1:200

Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-AMPK alpha (Thr172) Antibody detects endogenous levels of AMPK alpha only when phosphorylated at Threonine 172

Immunogen:

A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Threonine 172

Description:

AMPKA2 a protein kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.