Alternative Names:
APRF;Acute-phase response factor;Signal transducer and activator of transcription 3; STAT3; HIES;
Application:
WB IHC ICC/IF
Reactivity:
Human Mouse Rat
Dilution:
WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Human liver lysate; (3) Mouse heart lysate; (4) Rat brain lysate. |
Immunohistochemical analysis of paraffin-embedded rat brain, using STAT3 Antibody. |
Immunofluorescent analysis of Hela cells treated with IFNalpha, using STAT3 Antibody. |
Purification:
Affinity-chromatography
Specificity:
STAT3 Antibody detects endogenous levels of total STAT3
Immunogen:
A synthesized peptide derived from human STAT3
Description:
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
