Protein Maker is a mixture of ten (10) blue,orange,and greenstained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis(SDS-PAGE) and Western blotting.

SDS-PAGE band profile of the Protein Maker
Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.

Migration patterns of the Protein Maker in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns we

Features of Protein Maker:

• Size range—10 proteins spanning 10 to 180 kDa
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—color-coded bands of similar intensity for easy visualization
• Quality tested—each lot evaluated by SDS-PAGE and Western blotting
• Two reference bands—orange at 70kDa and green at 10kDa
• Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots