Alternative Names:

V5 tag

Application:

WB

Reactivity:

All

Dilution:

WB 1:3000~1:10000

Western blot analysis of extracts from v5-tag fusion protein, using V5 tag antibody.

Purification:

Affinity-chromatography

Specificity:

V5 tag antibody detects endogenous levels of C-terminal, internal, and N-terminal V5-tagged proteins.

Immunogen:

A synthesized peptide

Description:

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.