Alternative Names:

AKT2; Murine thymoma viral (v-akt) homolog-2; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolo

Application:

WB  IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000    IHC 1:50-1:200

Western blot analysis of Akt phosphorylation expression in Insulin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Threonine 308

Immunogen:

A synthesized peptide derived from human Akt around the phosphorylation site of Threonine 308

Description:

Akt2 an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.