Alternative Names:
EC 2.7.11.1
RAC-PK-alpha
Protein kinase B
PKB
C-AKT/EC 2.7.11.1
RAC-PK-beta
Protein kinase Akt-2
Protein kinase B, beta
PKB beta/EC 2.7.11.1
RAC-PK-gamma
Protein kinase Akt-3
Protein kinase B, gamma
PKB gamma
STK-2
Application:
WB IHC
Reactivity:
Human, Mouse, Rat
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200
Western blot analysis of Akt phosphorylation expression in heatshock treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Purification:
Affinity-chromatography
Specificity:
Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Serine 473
Immunogen:
A synthesized peptide derived from human Akt around the phosphorylation site of Serine 473
Description:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
