Alternative Names:

Tyrosine-protein kinase ZAP-70; ZAP70; SRK;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:1000 IP

Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.

Immunoprecipitation analysis of Jurkat cell lysates (ZAP-70 positive expression cell line)and Ramos cell lysates(ZAP-70 negative expression cell line) using ZAP-70 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

ZAP-70 Mouse mAb detects endogenous levels of total ZAP-70

Immunogen:

Purified recombinant human ZAP-70 protein fragments expressed in E.coli.

Description:

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development.Contributes also to the development and activation of primary B-lymphocytes.When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.