Alternative Names:

FAK; FADK; FAK1; FRNK; pp125FAK; PTK2;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200

Western blot detection of FAK in COS7,HUVEC,A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.

Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20℃ and using FAK mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

FAK Mouse mAb detects endogenous levels of total FAK

Immunogen:

Purified recombinant human FAK protein fragments expressed in E.coli.

Description:

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.