Alternative Names:

AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; HAMPKb; PRKAB1;

Application:

WB IHC ICC/IF IP

Reactivity:

Human,Mouse,Rat,Monkey

Dilution:

WB 1:1000 IHC 1:200 ICC/IF 1:100 IP

Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.

Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Immunofluorescent analysis of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).

Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

AMPKβ 1 Mouse mAb detects endogenous levels of total AMPKβ 1

Immunogen:

Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.

Description:

Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.