Alternative Names:

AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-PK-alpha; RAC-alpha serine/threonine kinase, kinase Akt1;

Application:

WB

Reactivity:

Mouse,Transfected

Dilution:

WB 1:2000

Western blot detection of AKT1 in 3T3 cell lysates using AKT1 mouse mAb (dilution 1:2000),with super ECL.Predicted band size:60KDa.Observed band size:60KDa.

Purification:

Affinity-chromatography

Specificity:

Akt1 Mouse mAb detects endogenous levels of total Akt1

Immunogen:

Purified recombinant human AKT1 protein fragments expressed in E.coli.

Description:

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.