Alternative Names:

AIK; ARK1; AYK1; Aurora-A; Aurora-family kinase 1; Aurora-related kinase 1; Aurora/IPL1-related kinase 1; BTAK; IAK1; STK15; STK6; Serine/threonine kinase 15;

Application:

WB ICC/IF

Reactivity:

Human,Monkey

Dilution:

WB 1:500 ICC/IF 1:50

Western blot detection of Aurora Kinase A in SW480 and COS7 cell lysates and using Aurora Kinase A mouse mAb (1:500 diluted).Predicted band size: 46KDa.Observed band size:46KDa.

Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using Aurora Kinase A mouse mAb (dilution 1:100).

Purification:

Affinity-chromatography

Specificity:

Aurora Kinase A Mouse mAb detects endogenous levels of total Aurora Kinase A

Immunogen:

Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli.

Description:

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.