Alternative Names:

CCAAT-box binding transcription factor; CTF; KIAA1439; NF-I/A; NF1-A; NFIA; Nuclear factor 1 A-type; Nuclear factor 1/A; TGGCA-binding protein;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:200 ICC/IF 1:200

Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.

Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

NFIC Mouse mAb detects endogenous levels of total NFIC

Immunogen:

Purified recombinant human NFIC protein fragments expressed in E.coli.

Description:

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.