Alternative Names:

CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; HP1 gamma; HP1Hs gamma; Modifier 2 protein;

Application:

WB IHC ICC/IF IP

Reactivity:

Mouse,Human,Monkey,Hamster,Rat

Dilution:

WB 1:1000 IHC 1:200 ICC/IF 1:200 IP

Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).

Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.

Purification:

Affinity-chromatography

Specificity:

HP1-γ Mouse mAb detects endogenous levels of total HP1-γ

Immunogen:

Purified recombinant human HP1-gamma protein fragments expressed in E.coli.

Description:

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.