Alternative Names:

Histone arginine methyltransferase CARM 1; Histone arginine methyltransferase CARM1; PRMT4; Protein arginine N methyltransferase 4;

Application:

WB IP

Reactivity:

Human,Mouse

Dilution:

WB 1:200-500 IP

Western blot detection of PRMT4/CARM1 in Hela,A431 and K562 cell lysates using PRMT4/CARM1 mouse mAb (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.

Immunoprecipitation analysis of Hela cell lysates using PRMT4/CARM1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

PRMT4/CARM1 Mouse mAb detects endogenous levels of total PRMT4/CARM1

Immunogen:

Purified recombinant human PRMT4/CARM1 protein fragments expressed in E.coli.

Description:

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.