Alternative Names:

Histone methyltransferase SMYD2; HSKMB; KMT3C; Lysine N-methyltransferase 3C; N lysine methyltransferase SMYD2; SET and MYND domain containing 2; ZMYND14.

Application:

WB

Reactivity:

Human,Mouse

Dilution:

WB 1:1000

Western blot detection of KMT3C / SMYD2 in 3T3,HT1080,A431 and Jurkat cell lysates and using KMT3C / SMYD2 mouse mAb (1:1000 diluted). Predicted band size: 50KDa.Observed band size: 50KDa.

Purification:

Affinity-chromatography

Specificity:

KMT3C/SMYD2 Mouse mAb detects endogenous levels of total KMT3C/SMYD2

Immunogen:

Purified recombinant human KMT3C / SMYD2 protein fragments expressed in E.coli.

Description:

Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.