Alternative Names:

DBCCR1; Deleted in bladder cancer protein 1 recursor; FAM5A;

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse,Rat,Monkey

Dilution:

WB 1:500 ICC/IF 1:200 IP

Western blot detection of DBC1 in HeLa,A549,Jurkat,C6,3T3 and COS7 cell lysates using DBC1 mouse mAb (1:500 diluted).Predicted band size:130KDa.Observed band size:130KDa.

Immunofluorescent analysis of HeLa cells using anti-DBC1 mouse mAb diluted 1:200.

Immunoprecipitation analysis of Hela cell lysates using DBC1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

DBC1 Mouse mAb detects endogenous levels of total DBC1

Immunogen:

Purified recombinant human DBC1 protein fragments expressed in E.coli

Description:

Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis.Inhibits SUV39H1 methyltransferase activity.As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.