Alternative Names:
EG5; HKSP; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP;
KNSL1; MCLMR; TR-interacting protein 5; TRIP5;
Application:
WB ICC/IF IP
Reactivity:
Human
Dilution:
WB 1:1000 ICC/IF 1:200 IP
Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa. |
Immunofluorescent analysis of Hela using Eg5 mouse mAb (1:200). |
Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb. |
Purification:
Affinity-chromatography
Specificity:
Eg5 Mouse mAb detects endogenous levels of total Eg5
Immunogen:
Purified recombinant human Eg5 protein fragments expressed in E.coli.
Description:
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
