Alternative Names:
KDM1;AOF2;BHC110;KIAA0601;LSD1; KDM1A;
Application:
WB ICC/IF IP
Reactivity:
Human,Monkey
Dilution:
WB 1:1000 ICC/IF 1:100 IP
Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa. |
 Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100). |
Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb. |
Purification:
Affinity-chromatography
Specificity:
KDM1/LSD1 Mouse mAb detects endogenous levels of total KDM1/LSD1
Immunogen:
Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
Description:
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
