Alternative Names:
Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9;
Application:
WB IHC ICC/IF FC
Reactivity:
Streptococcus pyogenes
Dilution:
WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:60
Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate. |
Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody . |
Purification:
Affinity-chromatography
Specificity:
CRISPR-Cas9 SP Antibody detects endogenous levels of total CRISPR-Cas9 SP
Immunogen:
A synthesized peptide derived from human CRISPR-Cas9 SP
Description:
The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
