Alternative Names:
Alpha fetoglobulin; Alpha fetoprotein precursor; FETA; HPAFP;
Application:
WB IHC ICC/IF IP FC
Reactivity:
Human
Dilution:
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Western blot analysis of AFP expression in HepG2 cell lysate. |
Purification:
Affinity-chromatography
Specificity:
AFP Antibody detects endogenous levels of total AFP
Immunogen:
A synthesized peptide derived from human AFP
Description:
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma .
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
